Breeding Journal: Berghia verrucicornis

Discussion in 'Breeding Journals' started by kbb0118, Sep 30, 2011.

  1. kbb0118

    kbb0118 Itty Bitty Sea Creatures
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    Breeding Journal DataSheet
    This first post should be updated regularly to include new information as events take place or changes are made to your system

    General
    Species: Berghia verrucicornis
    Social Structure: 2 adults
    Size of Individuals: 3/8" long when stretched
    Age of Individuals: Unknown
    Date added to Tank: 9/21/2011

    Broodstock Tank Details
    Size of Tank: 1 Gallon Kemps Ice Cream bucket - Mint Chocolate Chip flavor :) - floating in my 60 tall tank attached to my breeding system. Simple airstone for circulation.
    Substrate Details: None - no rocks/sand (they usually have bristleworms which will eat eggs)
    Filtration Details: None
    Water Changes: About 25% weekly.
    Water Temperature: 80 degrees F
    Lighting: Ambient room light / indirect and not bright
    Lighting Cycle: On at 12:30pm off at 9:30pm
    Other Tank Inhabitants: 1 Aiptasia is fed every other day

    Broodstock Feeding Details
    Food Types: Aiptasia
    Feeding Schedule: 1 anemone every other day or sooner if any adults are all white

    Spawning Details
    Date of First Spawn: 10/5/2011?
    Spawn Time of Day: Unknown
    Dates of Consecutive Spawns:
    Courtship Details: Two berghia stay in a Yin-Yang position. Mine were like that for about 1 day.
    Egg Size: approx 350 microns per egg
    Egg Color: White
    Egg Count: between 100-150 per coil

    Hatch Details
    Hatch Date: 10/13/2011 start
    Hatch Time of Day: evening
    # Days after Spawn: 10
    Larvae Description: tiny slugs only visible under microscope (see video)


    Larval Tank Details
    Temperature: 80 degrees F
    Size of Larval Tank: Specimin container hung on the 65 display tank, NO aeration
    Substrate Details: None
    Other Tank Decor: aiptasia
    Filtration Details: 25% water changes weekly
    Lighting: Ambient room light
    Lighting Cycle: on at 12:30pm off at 9:30pm
    Water Changes: 25% water changes weekly

    Larval Feeding Details
    Food Types: aiptasia anemones (clear colored)
    Feeding Schedule: continuous

    Metamorphosis/Settlement
    Date of Settlement Start: 10/14/2011
    Days after Hatch: 0
    Date of Settlement End: 10/14/2011
    Description of Fry: tiny slugs only visible under microscope (see video)

    Grow-Out Tank Details
    Temperature:
    Size of Grow-Out Tank:
    Substrate Details:
    Other Tank Decor:
    Filtration Details:
    Lighting:
    Lighting Cycle:
    Water Changes:
    Size at Transfer:
    Age at Transfer:

    Grow-Out Feeding Details
    Food Types:
    Feeding Schedule:

    Additional Information
    (No Pictures or Videos in the Section Please)
    Miscellaneous Information:
     
    #1 kbb0118, Sep 30, 2011
    Last edited: Oct 14, 2011
  2. reeflover

    reeflover I contributed!
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  3. OP
    kbb0118

    kbb0118 Itty Bitty Sea Creatures
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  4. Fragtastic Reef

    Fragtastic Reef We Dont Frag Around! Rare fish and more
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    Nice write up. Like how just keeping it very simple.
     
  5. OP
    kbb0118

    kbb0118 Itty Bitty Sea Creatures
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    Finally uploaded photos!

    Berghia adults culture
    [​IMG]

    Aiptasia culture:
    [​IMG]

    Egg coils freshly laid:
    [​IMG]

    Egg coils harvested and waiting in specimin box to hatch
    [​IMG]
     
  6. OP
    kbb0118

    kbb0118 Itty Bitty Sea Creatures
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    Microscope VIDEO (click on them)

    100X Video
    [​IMG]

    10X Video
    [​IMG]

    Pics

    100X
    [​IMG]

    10X
    [​IMG]
     
    #6 kbb0118, Oct 11, 2011
    Last edited: Oct 11, 2011
  7. reeflover

    reeflover I contributed!
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    Wow! Beautiful pictures. What diid you use to get them?
     
  8. OP
    kbb0118

    kbb0118 Itty Bitty Sea Creatures
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    Amscope B490B and my digital camera :biggthumpup:
     
  9. Dalailammy

    Dalailammy Amphipod First Class
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  10. OP
    kbb0118

    kbb0118 Itty Bitty Sea Creatures
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    Last night (estimated day 10 post spawn) one egg coil looked like it was starting to deteriorate on the outside (the thin membrane that creates the coil tube). So I did a technique I read about called "rasping" where you suck the egg coil in and out of a pipette to release the tiny eggs to hatch. Then I took some tiny dots and put them under the microscope. Some had hatched! Fun stuff!

    Video of one hatched (early Juvenile stage) and crawling around - check out all the tiny critter activity! What are those things??
    [​IMG]

    An empty egg:
    [​IMG]

    An unhatched one (Late veliger stage and metamorphosis):
    [​IMG]

    Same close to hatching one as above, magnified to 400X:
    [​IMG]








    Current pic of rearing vessel
    [​IMG]
     
    #10 kbb0118, Oct 14, 2011
    Last edited: Oct 14, 2011
  11. OP
    kbb0118

    kbb0118 Itty Bitty Sea Creatures
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    I seriously couldn't see anything in that specimin containter so I took a pipette and grabbed any specs I could find from the side of the container. I can't believe how tiny and FAST these guys are.

    I found this PDF paper on these guys. Very informative.
    http://www.frontiersinzoology.com/content/pdf/1742-9994-7-5.pdf

    VIDEO
    [​IMG]

    Photo 100X. You can see the cilia around the head!
    [​IMG]
     
  12. OP
    kbb0118

    kbb0118 Itty Bitty Sea Creatures
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    Oddly none of them seem to care for the Aiptasia at the moment. With my jewler's loupe and a bright lamp I can see there are hundreds zooming around on the side of the container. I hope that doesn't mean they are going to starve to death.
     
  13. Adam G

    Adam G Born to Reef!
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  14. wardda

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    Do you have any pictures of your aptasia grow out setup? Or is it the same as your Berghia setup, just another kemps ice cream bucket and air stone?
     
    #14 wardda, Nov 30, 2011
    Last edited: Nov 30, 2011
  15. OP
    kbb0118

    kbb0118 Itty Bitty Sea Creatures
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    It's just a bucket. See Post 5, 2nd pic.
     
  16. Baboo11

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    This is so cool! Interesting stuff, thanks for sharing it!:biggthumpup::mad:
     
  17. Baboo11

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    Oops! Doing this from my phone....didn't mean to hit the mad face!
     

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